08 August 2017 17 3K Report

"A properly designed guard column does not deteriorate the separation. As a matter of fact, a well designed and well packed guard column using the same material as in the analytical column is a small column extension that ADDS to the column plate count. It thus IMPROVES the separation. In some cases, a good sample preparation can eliminate the need for a guard column. However, in some cases it is virtually impossible to do so. This is the case when one is working with samples of biological origin: plasma, urine, meat, etc. Under these circumstances, a column contamination is practically unavoidable" 

http://www.chromforum.org/viewtopic.php?p=2086. 

More Mel Gibson's questions See All
Similar questions and discussions