Which methods are the fastest and easiest to do to save time. The LC-MS is broken for now and awaiting repair otherwise I would fragment the impurity with different m/z ionisation.
In the meantime as I wait for the response from the manufacturer what can I do in the meantime for analysing this impurity in the small molecule. How can I remove this impurity before the To void volume marker, or elute it well off past the total analysis time?. Any suggestions and experiences would be helpful. Can surfactants remove certain impurities or dilute them?. Are any of surfactants compatible with HPLC whether they are cationic, neutral or anionic?.
I want to centrifuge the solution and try to analyse it if there is a difference of nearly 100g/mol in molecular weight of the standard and the possible impurity.
I do not have smaller filter paper available to try and refilter a stock solution of that standard below 0.45 microns used for buffer salts filtration.
Also to switch on the HPLC visible lamp to analyse the molecule in the Vis-UV region to ensure that I know what peaks the molecule contains in both regions and to know how to change the elution of the impurity to prevent coelution with other analytes.
It's a solid in crystalline form that can be dissolved in highly organic solvent. I was thinking to do a melting point analysis on just the solid.
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