I read in Mass spec literature that buffer combinations are attempted. Are they only for gradient elution. Does this need mass spec to ionise the buffered solvent effectively. Will this cause the buffer to precipitate.
how does one make up such a buffer solution so simply?. Should I add formic acid to the ammonium acetate + ACN mixture already or add formic acid to 10ml of filtered ammonium acetate?.
Reverse phase is highly organic mobile phase at 90-97% ACN.
I did it!!!
Great idea on nucleoside and deoxyribonucleotides it gave me amazing performances, but I used amide column with an ehyl spacer (250x4.6,3um).
I can E.mail you the brand and some more details.
high temperature speed up the equilibration ; )
That's workable,however, U need be sure the MP won't influence your column efficiency.
Yes. I think you can use the combination. I do suggest to add the combination to the aqueous mobile phase.
Yes you may use salt for several reasons.
See why and how to prepare in the attached HILIC tips
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