Some sources claim that CUL2 is an important factor to fully activate the function of APOBEC3A (antiviral defence mechanism) against HPV- infections. CUL2 is important binding site for HPV16 E7 to stabilise A3A. HPV- 18 don't have this binding site.
Sources claim that highly methylated CpG- sites affect the prognosis of HPV- infection. And there are a difference between HPV- 16 and HPV-18.
01 May 2020 6,916 6 View
Hello, For my final project I am interested in conducting a multiple linear regression in which I have self-reported scores of autism, anxiety and alexithymia traits, and as a predictor I want...
29 April 2020 3,202 0 View
I've checked the ATCC, ALLCELLS, DSMA, Sigma and Thermofisher and they do not have any human preadipose cell lines.
27 February 2019 1,867 3 View
The main idea of modern recommender systems is to recommend you items similar to your profile. Your profile is calculated by your history (your favorite movies, items bought in online shops,...
08 July 2018 1,032 6 View
FYI - I am a professional classical vocalist, singing music of all centuries, including 21st century music. Very little of what I do is pure improvisation. Thank you. -Dr. Liana Valente
28 September 2017 2,740 6 View
We need to stain zebrafish brain slices for cell death markers so we are using Fluoro Jade C staining from Millipore (I'm using their protocol). The problem is that we have nothing, even in...
23 April 2017 7,317 1 View
I´m performing fmri. There is a persistent high signal on the occiptal region even using prescan normalize. When we analyze the data, all higher Z-scores are on this region, a d the paradigm does...
14 February 2016 6,450 2 View
Can someone tell me what is the minimal number of cells (for example CD8 T cells) that I have to put in a well culture. Is it realistic to measure the released cytokines from 2500 stimulated T...
31 January 2016 1,931 3 View
I have a feeling that many people don't know the answer of this question. So far I learnt, that the onset potential on the LSV is defined, so to say, as a potential at which the photo-induced...
18 January 2016 6,109 4 View
We extracted RNA from blood cells to analyze gene expression. We now have an aliquot with 500ng of the extracted RNA to make the reverse transcription (RT). The problem is that this 500ng are in a...
22 September 2015 7,781 14 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...
30 July 2024 9,824 2 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...
28 July 2024 6,515 1 View
Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...
28 July 2024 473 1 View
Please send me updates regarding the above-mentioned areas in the question mark?
28 July 2024 5,197 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View