We need to stain zebrafish brain slices for cell death markers so we are using Fluoro Jade C staining from Millipore (I'm using their protocol). The problem is that we have nothing, even in positive controls.The staining works perfectly in rat brain slices, but in zebrafish I see nothing. I couldn't get an answer from Millipore, so I want to know if anyone has already tried and was successful. Thanks!