I've checked the ATCC, ALLCELLS, DSMA, Sigma and Thermofisher and they do not have any human preadipose cell lines.
You should contact University of Cinicinnati regarding this particular cell line. http://www.ipo.uc.edu/index.cfm?fuseaction=technologies.results&item_number=106025
Dear Dr. Cerioni
You can try to request them from a lab that has published using them.
Like,
*Correspondence to: Nira Ben-Jonathan; Department of Cell and Cancer Biology; University of Cincinnati; 3125 Eden Ave.; Cincinnati, Ohio 45267-0521 USA; Tel.: 513.558.4821; Fax: 513.558.4823; Email: [email protected]
LS14 cells: A model for chemoresistance in liposarcoma
Article LS14 cells: A model for chemoresistance in liposarcoma
or
Case Number 106025 - LS14: A Human Adipocyte Cell Line
Description: We have cloned a novel cell line, named LS14, from a human liposarcoma tumor which expresses preadipocyte properties and proliferates robustly. This cell line is derived from spontaneously transformed cells and is not a result of transfection. The cells can be maintained and propagated indefinitely in a defined culture medium with a cell generation time of 72-96 hr. The cells are considered partially-differentiated preadipocytes and can be induce to terminally differentiate and accumulate lipid droplets following incubation in serum-free medium which contains a combination of adipogenic-inducing compounds. Within 8-10 days after induction of adipogenesis, 50-60% of the cells have undergone differentiation. These cells express many genes that are typical of primary human adipocytes, including leptin, adiponectin, lipoprotein lipase, hormone-sensitive lipase, fatty acid synthase, angiotensinogen, Glut4, AP2, TNF?, IL-6 and PPAR?. They respond to isoproterenol by increased lipolysis.
http://endo.endojournals.org/cgi/rapidpdf/en.2005-0989v1
For more information please contact Melissa Baines at 513-558-5274 or [email protected]
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