Is there a way to automatically gather surface area from a 3D model based on color/texture?

I have been constructing 3D models of large corals and I was wondering if there is a software that I can use to gather the area of live tissue vs dead/disease? I am aware there is a way by tracing...

17 December 2020 6,991 1 View

How can sea water be used as an input for green hydrogen production?

If you extract sea water, and filter it, would it be suitable as an input for green hydrogen production? From an electrochemical perspective, would this be the only process step required?

13 December 2020 2,665 8 View

Linear vs Supercoiled vs Nicked DNA on agarose?

I am pretty new to molecular biology techniques and just when I think I have it all figured out, something stumps me. This is how this gel (1.5% agarose) is loaded: 1. GeneRuler ready to use cat#...

06 October 2020 7,523 4 View

Can the Bray-Curtis Dissimilarity Index show differences in relative abundances of particular species. If not, which analyses can?

Hi all, I have a dataset of fish surveys containing counts of 80 or so species seen at 11 different reefs over a several year period. I would like to compare the community composition between...

13 May 2020 9,976 12 View

Can someone translate this paragraph into plain English?

Hi all, I'm looking into observational studies to try and better characterize treatment-resistant depression. I'm having trouble deciphering the paragraph in bold below, having read it multiple...

19 February 2020 9,823 2 View

Predicting the Age of home buyers - model based method?

All, I have a data-set which contains information on both the type of property an individual(s) purchased, along with some information on the individuals themselves. However, I am missing age...

19 February 2020 5,728 3 View

Techniques for removing electrosprayed nanoparticles from collector plates?

Hi, I am electrospraying polysaccharide micro particles directly onto a flat aluminium collector/foil surface. I am now trying to find a way to effectively remove them from the plate for...

04 February 2020 365 3 View

Best way to weigh hundreds of samples for a hyb ELISA?

We use a hybridization ELISA as a PK assay. We have hundreds of samples from different tissues of various treatments. Currently we're pre-weighing the tubes, then weighing the tubes with tissue...

16 August 2018 2,261 3 View

Does anybody know about a good mouse FPR2/FPRL1 antibody for flow cytometry?

24 May 2016 2,899 1 View

What are the known effects of Egyptian Goose on native bird populations in the UK/Europe?

12 January 2015 5,730 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View