Determining impact of NP-40 and Freeze/Thaw cycles
I am not sure if permission is needed but I am having trouble finding the holder of the copyright for the Survey of Perceived Organizational Support (SPOS) developed by Eisenberger et al. (1986),...
14 January 2024 6,153 1 View
I am looking for resources on viscosity vs temperature for HSA solutions used in IVF. There appears to be scant literature but perhaps I am not asking the correct way.
26 September 2023 452 0 View
Specifically, does anyone have experience of working with either or both Shimadzu Performance and Bruker Autoflex MALDI instruments and what is your opinion of instrument performance, servicing,...
03 October 2022 5,257 0 View
How do newer HPLC methods compare with post column ninhydrin detection on an ion exchange column?
03 October 2022 9,308 2 View
Virus titration. Detergents.
25 May 2022 9,789 1 View
We need to do our protein extractions in urea lysis buffer (recipe below) because SWI/SNF complex members bind very tightly to the DNA and won't show up on a western with just RIPA lysates. Is...
05 April 2022 2,220 2 View
Hi all, I'm currently in the full text review phase of an SLR on predictors / risk factors of treatment resistant depression. I'm including observational studies and RCTs. Virtually all of these...
21 March 2022 5,686 1 View
We recently purchased CAOV4 ovarian cancer cell line from ATCC which requires growth in Leibovitz's L-15 medium. This medium is designed to be used without CO2. Does anyone have experience using...
11 February 2022 2,856 0 View
I am looking to recreate foam bone. Can anyone comment on what type of foam is typically used in saw bones? For example I have used Polyurethane however the density if off as well as the feel and...
21 December 2021 1,705 1 View
Has anyone any experience in modeling and editing of anatomical models using 3-matic? If so have you used any competing softwares? Were there any big trade-offs for your work?
23 August 2021 6,358 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...
03 August 2024 2,366 0 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
