26 May 2022 1 10K Report

Virus titration. Detergents.

More Kristin O'Connor Barkus's questions See All
Does anyone know who I can email for permission to use the Survey of Perceived Organizational Support (SPOS)?

I am not sure if permission is needed but I am having trouble finding the holder of the copyright for the Survey of Perceived Organizational Support (SPOS) developed by Eisenberger et al. (1986),...

14 January 2024 6,153 1 View

Resources on the fluid properties of Human Serum Albumin?

I am looking for resources on viscosity vs temperature for HSA solutions used in IVF. There appears to be scant literature but perhaps I am not asking the correct way.

26 September 2023 452 0 View

Any opinions on Shimadzu vs Bruker MALDI TOF mass spectrometers?

Specifically, does anyone have experience of working with either or both Shimadzu Performance and Bruker Autoflex MALDI instruments and what is your opinion of instrument performance, servicing,...

03 October 2022 5,257 0 View

What is the best amino acid analyser on the market and why?

How do newer HPLC methods compare with post column ninhydrin detection on an ion exchange column?

03 October 2022 9,308 2 View

How do you utilize detergent in sonication of tissue homogenates designed to release virus or VLPs?

Determining impact of NP-40 and Freeze/Thaw cycles

08 June 2022 925 0 View

Protein quantification of cell lysate in urea buffer?

We need to do our protein extractions in urea lysis buffer (recipe below) because SWI/SNF complex members bind very tightly to the DNA and won't show up on a western with just RIPA lysates. Is...

05 April 2022 2,220 2 View

Which risk of bias tool should I use for a systematic literature review that contains mostly non-interventional studies?

Hi all, I'm currently in the full text review phase of an SLR on predictors / risk factors of treatment resistant depression. I'm including observational studies and RCTs. Virtually all of these...

21 March 2022 5,686 1 View

Alternative to Leibovitz's L-15 Medium for CAOV4 Cells?

We recently purchased CAOV4 ovarian cancer cell line from ATCC which requires growth in Leibovitz's L-15 medium. This medium is designed to be used without CO2. Does anyone have experience using...

11 February 2022 2,856 0 View

What is the foam used for trabecular bone in saw bones?

I am looking to recreate foam bone. Can anyone comment on what type of foam is typically used in saw bones? For example I have used Polyurethane however the density if off as well as the feel and...

21 December 2021 1,705 1 View

Mesh editing alternatives to 3-matic software by materialise?

Has anyone any experience in modeling and editing of anatomical models using 3-matic? If so have you used any competing softwares? Were there any big trade-offs for your work?

23 August 2021 6,358 1 View

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How to make Boehm titration ?

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Recovery Viurses from bacteria genome?

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Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

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Use of NC or NTC to descibe negative control?

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What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

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How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

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What is the fastest method should i use to determine TiO2 content for thermoplastic without using any high tech instrument?

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What is the effect of moisture of the atmosphere on titer of Karl-Fischer reagent (one-component)?

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What is the reason of appearance of 2 or 3 peaks in zeta potential analysis of sample?

Hello I have prepared metal nanoparticles and did zeta potential. Normally there is a single peak in sample but now there are 3 peaks. I prepared sample for analysis first I prepared 10 mg/mL...

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