How do newer HPLC methods compare with post column ninhydrin detection on an ion exchange column?
The L-8900 High-speed Amino Acid Analyzer is a high-speed liquid chromatography apparatus that employs columns filled with polystyrene divinylbenzene that has been altered by sulfonate groups. This is a very acidic cation-exchange resin with particles that are 3 m in diameter. In the protein hydrolysate analysis technique and the biological fluid analysis method, the mobile phases are sodium-citrate-type buffers for the 20 constituent amino acids and lithium-citrate-type buffer solution for the 40 constituent amino acids. Bottles of the buffer solutions that are available in stores can be directly linked to the gadget. In either analytical approach, a stepwise elution procedure is typically utilized, along with several kinds of buffer solutions, to elute a large number of components.
Every lab has got a HPLC System. Thus the best amino acid detection is to use specialised columns in various sizes. We collaborate with various column manufacturers and can offer systems that are engineered to your special needs. Just send us your personal needs (Costs, range size, resolution, global availability etc.) Please use the contact form in www.chromatographyshop.com and provide us with your country of location and company name.
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