I would like to concentrate and dry the ethyl acetate extract using rotary evaporator at 40 degrees under vacuum, but I am not sure under which conditions I am supposed to store the dried extract before applying it for TPC analysis and green silver nanoparticle synthesis. I would have difficulty removing the dried particles from round-bottomed flask of rotavap. Should I use a water bath instead to dry it?
As the most effective way to safely evaporate solvent from heat sensitive samples is to place the sample in a vacuum atmosphere. But at reduced pressure, the evaporation phase change occurs at a lower temperature, allowing the sample to be concentrated without damage from excessive heat. The common laboratory methods for solvent evaporation or sample concentration are lyophilization (also known as freeze drying), vacuum evaporation, nitrogen blow down and rotary evaporation depending on several factors— sample volume, sample quantity, required temperature range, and solvent(s) being evaporated. For your case, you can go ahead with water bath, as it is an excellent way to maintain samples at a set temperature, though when they are run at high temperatures or for long periods of time the water will of course evaporate, leaving behind dried particles in the round bottom flask.
Why dry the material when you’ll need to get it into the liquid phase for the reduction of your silver precursor?
thanks Anupma Thakur for your suggestion. Alan F Rawle , I would like to conduct TPC and TFC analysis, is it not supposed to be dry to mix with the suitable dissolving solvent? In my case I will be using 70% ethanol to dissolve the dried extract and standard. Can you please provide another alternative if I can use the same extract for both analysis (TPC and TFC) and synthesis of AgNPs. Thanks
Sorry Alan F Rawle TPC is Total Phenolic Content and TFC is Total Flavonoid Content.
Badria Al-Battashy
You need to find out if 70% ethanol will be suitable for your silver precursor or are you only referring to your analysis of the extract? I can't understand why you can't do your TPC and TFC (thanks for explaining the acronyms!) analyses in your liquid extract.
Mian Adnan Kakakhel
I am unsure what your comment means. I have no samples so I can keep them at whatever imaginary temperature I desire.
Alan F Rawle
The analysis is in terms of the mg standard per g of dried extract. So I have to obtain dried extract and measure it. Thanks again for your help, I will try to modify my procedure.
You can store your plant extract at 4 degrees for longer time. You can even store them at room temperature in dark.
Thanks Javed Iqbal . Do these same conditions apply for storage of prepared rutin stock solution?
My pleasure, But I usually store them at room temp in dark (lockers etc).
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