Which LCIA method is most suitable for assessing the impacts of nitrate leaching in groundwater (eutrophication )?

The eutrophication of groundwater by nitrate leaching is major environmental problem in Germany, especially in areas with intensive agriculture and high N-fertilizer inputs. In addition there are...

16 January 2021 5,182 7 View

How often should Mile Sven 1 (MS1) cells be passaged and at what cell density?

I'm looking for a culture protocol for MS1 cells (Mile Sven 1, ATCC: CRL-2279 ). This is an adherent endothelial cell line isolated from mouse pancreas. ATCC has a protocol, but it's lacking...

13 January 2021 4,175 3 View

GLMM in SPSS?

30 November 2020 2,716 4 View

Student's t-test or DeSeq2 for RNA-Seq data analysis?

Hi everybody, I am analysing RNA-Seq data of patient samples. One group of samples has a certain monoallelic deletion, the other group 2 copies of the wildtype gene. Now, I want to compare the...

28 November 2020 5,977 3 View

Indicated hydrogens in heterocyclic compounds - nomenclature?

Hello, I have a question to the IUPAC nomenclature of heterocyclic compounds. Usually hydrogen atoms in the ring system not attached to atoms connected by double bonds are denoted as indicated...

03 November 2020 3,313 3 View

Is there a good text/book on trend analyses?

Dear friends, I am looking for a book/text on trend studies and their statistical analysis. So far I found out that a trend study focuses on the same population with different individuals at...

22 August 2020 9,031 3 View

How often do you change a blade on a microtome?

Dear RG community, Histology related question: Can anyone advise on how many ffpe sections (estimated) can be cut using the same part of a microtome blade? The tissue I am sectioning is smooth,...

27 July 2020 1,297 3 View

What is the best wearable EEG recording device for research?

Hi there, I am searching a wearable EEG device with as many channels and the highest accuracy possible. I found some papers which reviewed/used the following devices, but still not sure which is...

19 May 2020 5,712 2 View

Is it possible to precisely evaluate a thickness of a NiP-Cr coating on a stailness steel with XRF?

Hello everybody, I have a qestion about the implementation of the XRF to determine the thickness of the NiP -Cr coating on a stainless steel (1.4305). According to the TDS of the base Material,...

24 February 2020 5,574 2 View

HPLC standard (mixture) for performance qualification test?

Has anyone experience in standards or standard mixtures for HPLC with UV detection for performing performance qualification tests? I am searching for e.g. a mixture of 2-3 compounds that is...

12 January 2020 537 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View