How do I stain extracellular DNA biofilm matrix?

More Tatiana Ramirez-Mora's questions See All

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Waht is the effect of isoelectric point on MB dye photodegradation by ZnO nanoparticles?

Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...

19 July 2024 4,868 1 View

Cloning 1334 bp insert into 6.1kb vector using HiFi DNA assembly/Gibson assebmly cloning ?

Hello, I am trying to clone 1334 bp insert into 6.1 kb vector using HiFi DNA assmebly/Gibson assembly kit. I am taking 100ng of vector and 1:5 molar ratio of vector: insert and 4 hrs of...

27 June 2024 9,652 2 View

How can I calculate the water molecules that are in a hydrogel sample?

I am looking for a way to calculate the number of water molecules that are in a hydrogel sample.

16 May 2024 7,064 3 View

Is there any published article about the effects of using a minor allele frequency filter on the quantity of missing data per individual?

I performed two RAD-seq assemblies using ipyrad. I filtered the outputted .vcf file of each of the two assemblies using vcftools to limit the maximum quantity of missing data per locus to 60%. To...

12 March 2024 2,136 0 View

Higher education acces and socioeconomic factors?

Articles about this topic, statistical methods and analisys for this. What socioeconomic factors are related with higher acces education?. Thanks.

06 December 2023 7,177 6 View

Why CMV promoter and SV40 promoter is used used in mammalian expression vectors?

I am having a doubt regarding CMV promoter and SV40 promoter in mammalian expression vector 1. Why CMV and SV40 promoters are used in mammalian expression vectors why not others? 2. There is a CMV...

05 December 2023 1,830 3 View

Cómo puedo subir a ResearchGate mi nuevo libro Psicoteología: la neurociencia de la fe (publicación pdf), para que sea pública?

Me gustaría subir a ResearchGate mi nueva publicación en el formato de pdf Psicoteología: la neurociencia de la fe, para la difusión y divulgación del contenido. Gracias.

05 November 2023 7,352 1 View

¿Cuáles son las estrategias empleadas para el fomento y evaluación de la competencia; Pensamiento Creativo en programas de arquitectura ?

El enfoque por competencias en educación, durante los últimos años ha alcanzado gran relieve en los diversos proyectos educativos, sobre todo, porque hay muchos modelos educativos que se han...

11 June 2023 3,550 2 View

Can I use only one sgRNA for my CRISPR Knock in?

Hello, I am trying to achieve the CRISPR KNOCK IN of HDR 1.7 kb in HEK293T will one sgRNA will be efficient to get a knock in or else should I use two sgRNAs?

19 May 2023 9,990 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Is it true that $\det(V(A))$ may be only $\pm 1$, depending on $n$, for the last symmetric tridiagonal matrix $A$?

One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...

08 August 2024 6,690 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View