Is running several one-way ANOVAs justifiable when an (appropriate) two-ways ANOVA does not reach statistical significance?

Hi, We have been discussing this topic on my lab for a long time. Is there any justification for reporting statistically significant results of several one-way ANOVAs when a previous and more...

29 January 2020 8,458 2 View

Choosing compound' doses for rats when only mice-related bibliography is available?

Hi all, We are planning to systemically deliver a synthetic pharmacological compound in rats. When looking for specific dosage and administration protocols, we only found studies with mice....

05 May 2019 3,644 5 View

TCID50 for Non-forming and Non-CPE virus?

Is there a possibility to calculate TCID50 for non-plaque forming virus, the virus I'm trying to titer is also known to be non-cytopathic, so my questions is: Is there a method of titers this sort...

15 April 2019 8,266 2 View

No title

10 March 2019 7,613 4 View

With SOLID185 microplane damage model. How do I solve my error in Ansys??

In the last weeks I have worked the damage model of the element SOLID65, but it has not allowed any results, so I decided to work with the element SOLID185, with the recommendations of the...

07 March 2019 5,662 5 View

Can someone help me with Immunofluorescence Assay for CSFV quantification?

I'm testing a protocol for viral quantification by IF; it is being standarizing from the fixation step, as a fixative choosen are Methanol (10mins) and 4% Formaldehyde-10%Methanol (15mins) (Is...

26 February 2019 3,020 1 View

What kind of body measures can I take in wasps to contrast differences between individuals from different nests?

27 January 2019 7,520 11 View

I need to extract dna from environmental biofilms from contaminated sites that solution recommend me to clean my samples?

biology

03 April 2017 9,126 0 View

Somebody can recommend me some Books?

I'll become a student (MSc by research in organic chemistry). I would like to know some useful books expecially for the "work in the laboratory" like spectroscopy, how to purify some reagents...

02 March 2017 2,429 0 View

What is the best way to recover low amounts of DNA from an agarose gel band?

10 November 2015 9,428 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How to find a correlation between coordinates in a curvilinear coordinate system?

Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...

28 February 2021 364 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View