Can anyone please tell me the best anti fungal agent that can be used for the removal of mold contamination from cell culture media and also the required concentration of the anti fungal agent per mL of media?
Hi Abhilash,
You can control the mold contamination in the cell culture media by adding commercially prepared or by manually using Antibiotic Antimycotic Solution (combination of 10,000 units/ml penicillin G, 10 mg/ml streptomycin sulfate and 25 μg/ml amphotericin). Penicillin and streptomycin will avoid the bacterial contamination; while amphotericin B will do it against fungi. Antibiotic solution should be prepared in higher concentration (preferably 100x) and can be used at 1x concentration for experimental propose.
I agree with Mr.Thomas Mohr, as retrieving the culture from mold contaminated is seriously not possible, as it will again leads contamination after few passage and sometimes may also change the nature of the cells.
I agree, try some antimycotics like amphotericin B. However, keep in mind that it might be cytotoxic in higher concentrations and may cause artifacts (e.g. non-specific activation of some immune cells etc.).
All in all, the best way is to prevent such contamination by sterile work or discard the culture and set up new one if possible.
Dispose of your cells and start with a fresh stock of cells that you know not to be contaminated. Using higher concentrations of antibiotics is not recommended. Apart from probable continued contamination, it can significantly alter cell signalling and alter their behaviour, potentially resulting in false results in your experiments.
Hello Abhilash,
I strongly discourage you from using any antibiotic or antifungal agents because, as mentioned before, it will alter your results.
I´d like to add that you also should clean your workbench, pipetors, laminar flow... everything which was in contact with your container. The reason is that spores spread easily in the environment of your lab.
Definitely. All of the are good answers. Depending on the purpose of your experiments I will advise you also to throw them and start again. The quality of your cells will determine the quality of your data. Thaw new cells and get the cells ready to use will just take you a week and a half, more or less.
Ask this question like that; means you can not take away the cells. I thought, maybe; there is a last stock. In this case, I have a proposal for you. InvivoGen the Plasmocin (Toulouse / France). This product has the use of both elimination and prevention doses.
This is product: http://www.invivogen.com/plasmocin
Good work.
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