Hello everyone,
I'm performing in vitro irradiation of GBM cells, but I wonder if its better to keep them on ice during the irradiation or if this is better to stay at RT° ?
Because I read some papers describing the effect of temperature change on the gamma H2AX level, and this is the protein that I want to check after by WB…
Thanks in advance
A. Henry
Maxim E Kuil
I would use a constant temperature RT is an option, but I guess 37 C reflects the "normal" condition of these cell better. On ice you will probably influence DNA repair significantly and this situation if far from normal in brain tissue.
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Elsie Magdalena Nolte
Room temperature (37 C) is best. Decreasing the temperature will decrease the DNA repair process in which H2AX plays a role. Good Luck!
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