Aurélie Henry

9 Questions 9 Answers 0 Followers

Questions related from Aurélie Henry

How to perform Electroporation Immunization with GFP-plasmid using BTX Agile Pulse ?

Dear All, I would like to set up DNA immunization in a mouse model, using the electroporation immunization (BTX Agile Pulse device). To start, I wanted to set up the parameters of...

22 September 2022 3,650 2 View

How to maintain and expanse brain tumor-derived lymphocytes in vitro?

Hi everyone, I'm trying to isolate lymphocytes from tumors resections (GBM) and to maintain them in culture. I would like also to expanse them to use for several experiments. Does someone has...

20 November 2017 3,386 3 View

Who is using RapID system for mouse identification?

Who is using the RapID system for identification of mouse/rats? Is it working well? The reading of the QRcode is easy and trustable? Does it hurt the skin of mice/rats? Thanks in advance

17 August 2017 9,129 1 View

Does it exist an application for in vivo follow up?

Hello everyone, I wondered if there is an application or a software available (for free or not) to monitor in vivo experiments, to register results and make the follow up of animals? Do you ever...

09 August 2017 6,992 2 View

Which microplate reader to choose?

Hello everyone, I'm looking to order a microplate reader, fluorometer and spectrophotometer. According to your experience with this kind of machine, which brand/model would you advice me...

02 December 2016 769 7 View

Can IP injection of mouse cancer cells induce inflammation in mice ?

Hello everyone, I performed IP injection of mouse cancer cells (GL261, 75.000 cells) in C57bl/6 mice. However, three weeks after, mice started to be moribund and necessitated to be sacrificed....

03 November 2016 4,466 3 View

Do we need Matrigel for SW48 colon cancer cells SC-injection?

Hello everyone, I'm starting working with SW48 colon cancer cells in vivo, and I wondered if Matrigel is needed for a subcutaneous injection. And, how much cells do you generally inject? Thanks...

31 August 2016 1,962 3 View

Why NOD-Scid do not tolerate irradiation while nude mice do ?

Hello everyone, I wondered why NOD-Scid mice are suffering of irradiation exposure and nude mice not? I performed half brain irradiation in NOD-Scid model and after 5 days, all mice started to...

15 June 2016 9,880 5 View

How do I perform in vitro irradiation on GBM cells?

Hello everyone, I'm performing in vitro irradiation of GBM cells, but I wonder if its better to keep them on ice during the irradiation or if this is better to stay at RT° ? Because I read some...

16 April 2015 3,583 3 View