We are working with a bovine SV40-LargeT-antigen fetal hepatocyte-derived cell line. Can anyone give information on how to induce bovine serum albumin production?
Thank you very much!
It completely depends on the differentiation stage of the cells you are using.
Usually continuous replication state of hepatocytes reduce its differentiation. You can overcome that by reducing the serum in the medium, or using serum free culture medium.
Also SV40-T usually induce chromosomal aberrations, and this will of course affect cellular phenotype.
Hussein H Aly, serum free culture/serum reduction might force cells to stay in G0 state with higher differentiation, is that correct?
I found a publication which says using laminin coated culture plates might induce albumin production in primary hepatocytes, cells cultured on collagen I coated flasks will rapidly lose albumin production:
Caron, J. M. (1990). Induction of albumin gene transcription in hepatocytes by extracellular matrix proteins. Molecular and Cellular Biology, 10(3), 1239–1243. doi:10.1128/MCB.10.3.1239.Updated
Is anybody familiar with induction of albumin production by coating cell culture plates or addition of laminin to cell culture medium?
Alexander, yes serum reduction force cells to stay G0 with higher differentiation,
Regarding the effect of extracellular matrix, I have no idea, but the culture condition also affect the cellular differentiation, and albumin production, like 3D cultures and bioreactors.
Best wishes
Hussein H Aly, thank you very much!
Do you have a protocol for serum free hepatocyte culture or 3D culture? Is there any good literature on higher differentiation of heptocytes in 3D or 2D sandwich culture?
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