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Questions related from Alexander Gleich
For experiments on induction of the Cytochrome P450 system in cattle, we need to order the dioxins TCDD and TCPOBOP. The question is: Do you have made good experiences with a particular...
01 January 2016 7,323 1 View
We are planning to measure bile acid production in an bovine hepatoyte-derived cell line at our group. In pre-experiments, our cells mainly produced taurine conjugated bile acids, probably because...
07 July 2015 2,903 2 View
Currently we are planning qPCR gene expression studies of the biotransformation system of our hepatocyte-derived cell line from cattle. What would be suitable CYP inducers beside beta-naphtoflavon...
07 July 2015 8,205 4 View
for conducting experiments with a bovine hepatocyte cell line, I am looking for a good protocol to establish an EROD assay for measurement of CYP1A1 activity. Is there anyone who could help me...
03 March 2015 793 5 View
We are working with a bovine SV40-LargeT-antigen fetal hepatocyte-derived cell line. Can anyone give information on how to induce bovine serum albumin production? Thank you very much!
01 January 2015 5,902 4 View
Could anyone provide me a protocol for endotoxin determination of medium and buffer for RAW 264.7 macrophages? Thank you very much!
09 September 2014 7,142 3 View
I'm currently looking for the most important CYPs of bovine hepatocytes. Which ones would you suggest for detection by RT-PCR?
08 August 2014 6,735 3 View
We have established and characterized a bovine SV40-Large-T-transduced cell line. For information on how many population doublings the cell line does until crisis, I want to calculate it. Is it...
03 March 2014 1,736 5 View
