Where do you order dioxins such as TCDD and TCPOBOP and do you use solutions or solids?

For experiments on induction of the Cytochrome P450 system in cattle, we need to order the dioxins TCDD and TCPOBOP. The question is: Do you have made good experiences with a particular...

31 December 2015 7,277 1 View

Any advice about bile acid production in bovine hepatocyte cell line?

We are planning to measure bile acid production in an bovine hepatoyte-derived cell line at our group. In pre-experiments, our cells mainly produced taurine conjugated bile acids, probably because...

06 July 2015 2,807 2 View

What are possible CYP inducers for a bovine hepatocyte cell line?

Currently we are planning qPCR gene expression studies of the biotransformation system of our hepatocyte-derived cell line from cattle. What would be suitable CYP inducers beside beta-naphtoflavon...

06 July 2015 8,164 4 View

Can someone recommend a protocol for EROD assay (CYP1A1)?

02 March 2015 740 5 View

How do I induce albumin production in fetal hepatocytes?

We are working with a bovine SV40-LargeT-antigen fetal hepatocyte-derived cell line. Can anyone give information on how to induce bovine serum albumin production? Thank you very much!

31 December 2014 5,852 4 View

How do I conduct endotoxin determination of medium and buffer for RAW 264.7 macrophages?

Could anyone provide me a protocol for endotoxin determination of medium and buffer for RAW 264.7 macrophages? Thank you very much!

08 September 2014 7,045 3 View

How do I properly calculate population doublings in a cell line for the generation of a graph?

We have established and characterized a bovine SV40-Large-T-transduced cell line. For information on how many population doublings the cell line does until crisis, I want to calculate it. Is it...

02 March 2014 1,648 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View