We are planning to measure bile acid production in an bovine hepatoyte-derived cell line at our group.
In pre-experiments, our cells mainly produced taurine conjugated bile acids, probably because of high taurine content in FCS (measured by LC/MS-MS). Now we are looking to add defined amounts of glycin and taurine. Is there any literature on this or do you have any hints how to conduct this experiment?
You can please refer to the following links to get certain valuable details:-
1. http://www.jbc.org/content/264/18/10384.full.pdf
2. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070591/
Dear Alexander,
We've used hepatocytes isolated from male dairy calves were used in monolayer culture. An effective method has been developed for quantitative determination of six bile acids including lithocholic acid (LCA), deoxycholic acid (DCA), chenodeoxycholic acid (CDCA), hydodeoxycholic acid (HDCA), cholic acid (CA) and ursodeoxycholic acid (UDCA) in bovine liver by gas chromatography-chemical ionization/tandem mass spectrometry (GC-CI/MS/MS). Satisfactory results were obtained when bile acid derivatives of LCA, CDCA, HDCA, and UDCA were determined with total ion chromatograms (TIC) while DCA and CA were determined with extracted ion chromatograms (EIC), respectively. The detection limits (S/N=3) for six bile acids in biological tissues were ranging from 0.40 to 1.6 ng/mL and the recoveries indicated that the proposed method was feasible for the determination of trace bile acids in the biological samples studied. In addition to DCA and CDCA, trihydroxy bile acid, CA, are the major bile acids in bovine liver.
Best wishes,
Ilya
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