How do I find where Agilent mRNA microarray probes bind?

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How to solve the problem of unconscious teeth grinding during sleep at night?

One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...

08 August 2024 1,366 5 View

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to send my account link and how to make my account public?

Nothing

01 August 2024 755 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Should Coefficient of Variation (CV) in a biological data necessarily be below 30%?

In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...

27 July 2024 201 0 View

Non-parametric version of the wo-way repeated measures ANOVA?

Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...

24 July 2024 3,284 6 View

Is possible to edit a gene present in a plasmid by Crispr Cas9?

Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...

22 July 2024 7,073 2 View

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

To what extent are artificial intelligence techniques used to enhance science learning in schools in the Arab Gulf countries???

I want researchers who specialize in studying artificial intelligence in the field of science education and those related to it to give me information about the application of the...

18 July 2024 8,320 0 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

How to build my own lab made four point probe set up?

Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...

30 July 2024 906 2 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

AGILENT HPLC 1100 SERIES , any expert available please?

AGILENT HPLC 1100 SERIES operation assistance needed.

25 July 2024 10,045 2 View

Chromosomal spreads are shrunken after fluorescence in situ hybridization (FISH), why?

Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...

08 July 2024 8,687 3 View

Can I design a probe for taqman qpcr in antisense?

I have a target of sequences for primer design for qPCR with taqman. The issue is that when I design a sense probe the probe makes dimers with forward and reverse with a very high probability at...

16 June 2024 1,506 1 View

Problems with designing syber-green primers for lnc_RNA GAS5?

Hi all, you know this lncRNA has 12 exons and 50 isoforms that just have similar sequences in exon 12. Coud someone recommend me primers for this exon with no need of probes?

15 June 2024 8,055 0 View

Can holes for pressure probes in journal bearings modify the original pressure distribution?

It is very common to measure the pressure distribution inside the bearings with pressure probes connected to the oil film through holes in the bush. Once filled and pressurized, the probe lines...

12 June 2024 1,936 0 View

Need to obtain concentration of DNA/RNA?

I need to use a photometer, so i have Cary Agilent and i would like to know what kind of cuvettes and its dimensions and volume i have to order. It's about RNA and DNA samples. Which are you...

11 June 2024 712 1 View

Why am I having huge shifts in reference genes Cq's?

I was recently performing a great deal of qPCRs on adipose tissue derived RNA. I had experimental samples from sick, and control samples from healthy individuals. I used the same chemistry for all...

04 June 2024 6,924 0 View