What is the technique and skills required to culture E14 (mESC) to keep it passing without differentiation?
Addition of Leukemia inhibitory factor (LIF) to the growth medium will keep mESC cells in an undifferentiated state.
Check if your mESC need a feeder layer for growing and change medium every day because the level of LIF in the media decresaes.
Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...
03 February 2021 3,039 1 View
Which plasmids will be the best for making mRNA vaccine? What are the points for consideration when choosing suitable plasmid for such purposes?
02 February 2021 9,092 1 View
Hello, I am new to flow cytometry, and was just using a sample cell population to train with the technician. The cells were PAC-1 cells split into four groups. Unstained, transiently transfected...
28 January 2021 969 2 View
I'm looking to produce some phi correlations and I'd rather not do it manually, but I can't find a library for R that includes phi.
18 January 2021 2,808 9 View
Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...
13 January 2021 8,336 4 View
I have a SEM model that I'm having difficulty interpreting. It has one direct effect with a Beta/standardised regression weight that appears to be high (0.80) and significant (p
31 December 2020 7,604 6 View
I've produced a violin plot for a point-biserial correlation. Main title is there, x and y are labelled, but I want to add a custom text box with the correlation coefficient and significance...
26 December 2020 4,480 4 View
I understand several normal DNA intercalators, such as ethidium bromide, acridine, SYBR et al. would emit fluorescence. Is there any kind of DNA intercalator that do not have fluorescence?
25 December 2020 688 3 View
I've just run a series of point biserial correlation tests in R between whether or not characters were assigned national identities, and attributions given to their behaviours - results shown in...
17 December 2020 9,825 4 View
What I did (unpublished yet) is extract several (e.g. 16) mask features within Mask R-CNN from multiple images, construct a distance metric to compare them to predict the image class. Each set of...
13 December 2020 9,504 2 View
02 March 2021 3,060 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
28 February 2021 9,936 2 View