How do I culture E14 (mESC) to keep it passing without differentiation?

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VASP recognize my insulating alloy structure as not insulating?

Hi, Recently I was running VASP to calculate the spontaneous polarization of my alloy structure. I followed the instructions in this PDF wrote by Dr. Nabin (Nabaraj) Pokhrel, below is my INCAR...

07 August 2024 486 5 View

What are the frameworks or methodologies to examine written academic ELF?

What should be the frameworks or methodologies to examine written academic ELF? I want to explore the linguistic features of written ELF in research articles.

23 July 2024 4,800 1 View

How to analyse response rates of a questionnaire?

I have developed a questionnaire. I want to try and analyse question drop-off. There is a clear negative correlation between question number and percentage of respondents who answered that...

07 July 2024 9,435 1 View

Can anybody recognize the virus on potato plants?

Infected potato plant was negative for PVY, PVX, PVM, PVS. What can it be?

19 June 2024 6,739 3 View

Is the preference for more established researchers by reputable academic journals leading to infrequent publishing of Bachelor's degree theses?

I think, for a long time, there has been a tendency for journals to favour work from more established researchers or institutions, thus potentially overlooking quality undergraduate research. This...

16 June 2024 6,801 3 View

How do you generate recursive proofs over time series data?

What are the recommended recursive algorithms to generate proofs of time variant and time invariant properties from a time series data. Approaches like recursive statistical tests, symbolic time...

08 June 2024 4,449 0 View

What is the ratio between the density of the bacterial population during anaerobic and aerobic growth of one species?

What is the relationship between the maximal density of a bacterial population during anaerobic and aerobic growth for a specific species - a facultative anaerobe?

01 June 2024 4,950 4 View

Why do Spanish slugs (Arion vulgaris) prefer to feed on the most poisonous or spicy plants?

Spanish slugs choose to feed on some of the most poisonous and spicy plants. Do they become toxic to potential predators due to this? Are there any publications available on this topic?

28 May 2024 3,929 0 View

Low-yield of Cas9 plasmid after maxi-preparation. Anyone has the same issues?

Hi~ We are making different Cas9 plasmids such as regular spCas9, base editing and prime editing under different promoter such as CMV and CAG. During selection of colonies, we always get a good...

22 May 2024 7,336 0 View

How build Forest plot in STATA?

(STATA) Hello everybody. I would like to build a Forest Plot graph that includes all this information from the table. I am unable to build the commands to include this information in the chart....

11 May 2024 6,944 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View