Is HUVEC cells a suitable choice as endothelial cell for in vitro tissue matrix study?

For In vitro tissue scaffold study I have to choice a suitable cell line. I have searched ATCC and found the HUVEC cell. Is it appropriate?

07 August 2014 6,488 3 View

Problem with A549 cells and radiation exposure and cell viability tests

We have irradiated A549 cells in 96 well-plate with 2Gy, 4 Gy 8Gy and 16 Gy doses and after 5 or 7 days the viability of cells decreased only to the 60 % with XTT test. It means these radiation...

01 February 2013 5,495 17 View

Dissolving iron-nickel, silver-tin, and copper-zinc nanopowders

How can I dissolve iron-nickel, silver-tin, and copper-zinc nanopowders to treat biological samples and also characterize them? I've purchased metal alloy nanpowders to treat cancer cells but I...

06 July 2012 4,442 3 View

I used 40 nm gold nanoparticles in cell culture. How can I view them inside the cell?

Is it suitable to use scanning electron microscope (SEM), or is there any antibody to show gold nano inside the cell?

06 July 2012 8,749 15 View

how can I irradiated cells and then apply MTT tests is it possible to first irradiate them in empty plate then collect and cultured them in MTT plates

cell culture how can be irradiated the cells and then apply MTT or another Elisa tests

04 May 2012 2,211 3 View

We purchased 40nm diameter gold nanoparticles, and in 1ml 7,2e10 particle count how can we obtain the nanomolar concentration?

Nanoparticle research

03 April 2012 5,354 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View