Is HUVEC cells a suitable choice as endothelial cell for in vitro tissue matrix study?

For In vitro tissue scaffold study I have to choice a suitable cell line. I have searched ATCC and found the HUVEC cell. Is it appropriate?

07 August 2014 6,488 3 View

Problem with A549 cells and radiation exposure and cell viability tests

We have irradiated A549 cells in 96 well-plate with 2Gy, 4 Gy 8Gy and 16 Gy doses and after 5 or 7 days the viability of cells decreased only to the 60 % with XTT test. It means these radiation...

01 February 2013 5,495 17 View

I used 40 nm gold nanoparticles in cell culture. How can I view them inside the cell?

Is it suitable to use scanning electron microscope (SEM), or is there any antibody to show gold nano inside the cell?

06 July 2012 8,749 15 View

How do I convert XTT absorbances to OD and finally to per cent inhibition?

We are doing ELISA for XTT and can get absorbances but can not calculate ODs or per cent inhibition. Can somebody help?

06 July 2012 4,435 0 View

how can I irradiated cells and then apply MTT tests is it possible to first irradiate them in empty plate then collect and cultured them in MTT plates

cell culture how can be irradiated the cells and then apply MTT or another Elisa tests

04 May 2012 2,211 3 View

We purchased 40nm diameter gold nanoparticles, and in 1ml 7,2e10 particle count how can we obtain the nanomolar concentration?

Nanoparticle research

03 April 2012 5,354 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View