Problem with A549 cells and radiation exposure and cell viability tests

We have irradiated A549 cells in 96 well-plate with 2Gy, 4 Gy 8Gy and 16 Gy doses and after 5 or 7 days the viability of cells decreased only to the 60 % with XTT test. It means these radiation...

01 February 2013 5,495 17 View

Dissolving iron-nickel, silver-tin, and copper-zinc nanopowders

How can I dissolve iron-nickel, silver-tin, and copper-zinc nanopowders to treat biological samples and also characterize them? I've purchased metal alloy nanpowders to treat cancer cells but I...

06 July 2012 4,442 3 View

I used 40 nm gold nanoparticles in cell culture. How can I view them inside the cell?

Is it suitable to use scanning electron microscope (SEM), or is there any antibody to show gold nano inside the cell?

06 July 2012 8,749 15 View

How do I convert XTT absorbances to OD and finally to per cent inhibition?

We are doing ELISA for XTT and can get absorbances but can not calculate ODs or per cent inhibition. Can somebody help?

06 July 2012 4,435 0 View

how can I irradiated cells and then apply MTT tests is it possible to first irradiate them in empty plate then collect and cultured them in MTT plates

cell culture how can be irradiated the cells and then apply MTT or another Elisa tests

04 May 2012 2,211 3 View

We purchased 40nm diameter gold nanoparticles, and in 1ml 7,2e10 particle count how can we obtain the nanomolar concentration?

Nanoparticle research

03 April 2012 5,354 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View