Is HUVEC cells a suitable choice as endothelial cell for in vitro tissue matrix study?

For In vitro tissue scaffold study I have to choice a suitable cell line. I have searched ATCC and found the HUVEC cell. Is it appropriate?

07 August 2014 6,488 3 View

Problem with A549 cells and radiation exposure and cell viability tests

We have irradiated A549 cells in 96 well-plate with 2Gy, 4 Gy 8Gy and 16 Gy doses and after 5 or 7 days the viability of cells decreased only to the 60 % with XTT test. It means these radiation...

01 February 2013 5,495 17 View

Dissolving iron-nickel, silver-tin, and copper-zinc nanopowders

How can I dissolve iron-nickel, silver-tin, and copper-zinc nanopowders to treat biological samples and also characterize them? I've purchased metal alloy nanpowders to treat cancer cells but I...

06 July 2012 4,442 3 View

I used 40 nm gold nanoparticles in cell culture. How can I view them inside the cell?

Is it suitable to use scanning electron microscope (SEM), or is there any antibody to show gold nano inside the cell?

06 July 2012 8,749 15 View

How do I convert XTT absorbances to OD and finally to per cent inhibition?

We are doing ELISA for XTT and can get absorbances but can not calculate ODs or per cent inhibition. Can somebody help?

06 July 2012 4,435 0 View

We purchased 40nm diameter gold nanoparticles, and in 1ml 7,2e10 particle count how can we obtain the nanomolar concentration?

Nanoparticle research

03 April 2012 5,354 2 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View