How do I co-culture MSCs and colon cancer cells?

More Jasmin S. Nurkovic's questions See All

Positive control for Malachite Green ATPase assay?

I am trouble shooting a malachite green ATPase assay using a kit from Sigma (MAK307) for a cytosolic protein that converts ATP --> ADP + Pi and am wondering what cheap + commercially available...

30 March 2024 9,659 1 View

What is Plasma Insulin and why should it be used to test the transition from Gel Separatory Serum tubes to Lithium Heparin Gel tubes?

In the Journal "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory" the researchers found discrepancies in regard to the effects of plasma...

25 February 2024 9,949 2 View

How do we determine the optimal dosing regimens for antibiotics used in combination therapy against drug-resistant bacteria?

The optimal dosing regimen for antibiotics used in combination therapy against drug-resistant bacteria should be determined through careful consideration of these factors, along with ongoing...

23 February 2023 6,965 2 View

How accuracy repeatability and reproducibility?

We are now conducting Inter-laboratory test program. Would like to evaluate the Accuracy and Precision of each lab. For accuracy, we will use z-score. May I know how to calculate the Precision...

26 December 2022 3,310 1 View

Can you thaw and refreeze human ascites?

Hi everyone, I'm troubleshooting using frozen ascites for different techniques i.e. cell culture and immunoblotting. However, when the ascites specimens were collected they were put at -80 C in...

07 November 2022 6,487 0 View

NK cell expansion protocol?

Hi all, It's my first time culturing NK cells, not only mine but also the first time it is being done in my lab so I am kind of on my own. Does anyone have a step-by-step protocol they could...

23 March 2022 6,008 1 View

Can you use Human Serum Albumin (HSA) instead of Human Serum (HS) in NK cell culture media?

I'm using the Miltenyi Biotec NK MACS medium however the protocol says to use 5% AB Serum, is it possible to substitute the HS for HSA? IL-2 and IL-15 will be added to the media to help with NK...

22 March 2022 6,194 3 View

No DAPI seen after using "ProLong™ Gold Antifade Mountant with DAPI" ?

I have been using Prolong Gold with DAPI for my IF staining. However the last few times no DAPI signal has been showing up. Only problems I can think of is that I let the gel curate at 4C and...

21 July 2021 8,349 1 View

Do you know how to bond PDMS on sapphire wafer with plasma O2 please?

we try top bond two little pieces of pdms on sapphire wafer. These are the inlets of flexible tubes to a microfluidic channel located under the sapphire

28 April 2021 4,641 0 View

How do other researchers score the child behavior checklist?

We are using the Child Behavior checklist for research purposes, and wanted to know how to score the output. Is anyone familiar with a manual we can purchase that would inform us how to score...

15 March 2021 5,284 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Aftab Ahmad

What is purpose of this experiment? I think you have to culture them according to your experimental requirements

Jasmin S. Nurkovic

The aim is to investigate the influence MSCs to propagation and migration colon cancer cells.

Di Liu

You may use a transwell(8um) and Brdu label to observe cell migration and propagation, respectively.