How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Can someone help me with tissue histology of mice?

I've been trying to assess the efficacy of a drug that I've purified in mouse model. I've induced mammary tumors in balb/c mice and treated them with my drug. I've harvested the tumors, and other...

16 December 2020 3,515 3 View

Can anyone help to get this research paper on OCB?

Turnipseed, D & Murkison, G (2000). Good Soldiers and Their Syndrome: Organizational Citizenship Behavior and the Work Environment, North American Journal of Psychology 2(2)

11 November 2020 7,488 3 View

What are some landmark texts and papers on agroecology we need to follow for a clear understanding?

We often read a lot about sustainable agriculture or conservation agriculture (or recently, climate-smart agriculture) but it is often difficult (especially for early-stage researchers) to fathom...

08 April 2020 9,212 10 View

Can anyone share me a better image of the attached map? In which book the map was originally published?

The map is reproduced in Eaton, Richard M. The Rise of Islam and the Bengal Frontier, 1204-1760. Berkeley: University of California Press 1993; reference given are: Barrie M. Morrison,...

13 August 2019 1,485 2 View

Does 4T-1 cells induce ascites in mice?

I am new to in vivo research. I have tried to induce solid tumors in mice by injecting 4T-1 cells in the mammary fat pad, which is an established procedure. While i mostly ot solid tumors, 2 or 3...

18 June 2019 973 3 View

How to solve problems arising from choosebank() command in R?

I am using seqinr package in R. But when I am giving the command choosebank() to find out the existing databases or commands like choosebank('swissport") to open any specific database, R is...

15 April 2019 1,541 3 View

What is the scope of Fortran in machine learning?

I intend to use ML for molecular dynamics simulations and want to know of packages available in Fortran. Would it be more efficient to use some other language instead for the long run?

09 January 2019 8,295 2 View

Which regression/multivariate technique to apply if dependent var is continuous scale, cant be categorised, non-normal, Indep Variables dichotomous?

02 September 2018 1,981 2 View

For droplet-based sc-RNA sequecing data, library size varies across cells. How does one tackle this when calculating statistical variance for genes ?

To calculate the statistical quantities like variance or mean expression of a gene across cells, it is unfair to compare expression counts of a say gene A, if we know different cells has not be...

14 July 2018 10,016 2 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

Is MOPS buffer needed for in vitro horizontal gene transfer in thr coculture of two different pathogenic bacterial species?

Actually i am working on possibilities of horizontal gene transfer of resistant gene in broth coculture of two different types of bacteria, and the protocol i follow involves the use of MOPS...

19 January 2021 4,566 2 View

How can I insert antibody sequences into yeast cell to achieve stable and large quantity production?

I have the sequence of the light chain and heavy chain of a certain antibody. I would like to know how to express this antibody in yeast cells to achieve stable and large quantity production. It...

04 January 2021 9,669 2 View

Generate a structure file through PGDSpider?

Hi, I am trying to generate a structure file through PGDSpider, for a diploid organism, where each locus is in two consecutive columns in one row, rather than data for each individual be stored...

29 December 2020 5,169 1 View

How to prediction non-visible parts of microscopic pictures?

Dear all, I like to ask if someone has experience with evaluating pictures towards the prediction of non-visible parts. I have confocal-laser-scanning microscopic pictures of yeast cells and want...

28 December 2020 4,309 11 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

Any recommendations for a good CRISPR/Cas9 commercial system for functional genomics of fungi/yeast?

I look forward to implying a good CRISPR/Cas9 system with a selective maker for functional genomics of important genes from environmental fungi and yeast.

22 December 2020 1,432 2 View

Can I inactivate restriction enzymes after freezing the digestion product?

I performed a digestion that ended up at midnight, but forgot to inactivate the enzymes (HaeIII and RSAI) before freezing the products. I plan to run a gel to differentiate yeast isolates. Can I...

09 December 2020 3,551 3 View