Diptarka Dasgupta

9 Questions 4 Answers 0 Followers

Questions related from Diptarka Dasgupta

How to find ITS region of an organism from genome data?

I have genome data of yeast and want to find out it's ITS region. Can anyone suggest any tool or method to find out the ITS region?

03 April 2023 6,740 2 View

How to add activated carbon and yeast extract in my data inventory for Life cycle analysis using SimaPro?

I want to carry out life cycle analysis of a process using SimaPro software. My process involves the use of both yeast extract as a nutrient for fermentation and activated carbon for a...

03 April 2019 904 1 View

Is enzyme activity the rate of reaction?

I am trying to determine kinetic constants for my reaction. I have the enzyme activity in IU/ml. The unit of activity indicates one micromole of product formed per unit time. If we have to plot v...

06 July 2015 7,664 3 View

Anyone familiar with assembling in velvet queries?

I am trying to carry out de novo assembly of a genome of interest. I have some queries regarding operation of velvet tool. When the k mers are selected and hash table is made, does velvet know...

15 April 2015 7,209 1 View

How do I check the ploidy in yeast?

I have whole genome sequence data of my organism of interest. Is it possible to identify whether the organism is diploid or haploid from the data? Is there any such tool available?

30 March 2015 8,159 1 View

Could anyone tell me the inhibitors for an enzyme as described below?

Could anyone tell me the inhibitors of amino acids tyrosine, lysine, aspartate and histidine and conc of the same? I need to carry out inhibitor studies of these residues involved in active site...

02 March 2015 184 8 View

Can anyone tell me the procedure for xylitol estimation spectrophotometrically?

Is there any spectrophotometric assay for xylitol determination? I have searched on net but not been able to find the same.

28 January 2015 7,116 2 View

Can anyone provide me with a protocol for RT-PCR?

Can anyone provide me link of any paper to carry out RT-PCR for testing gene overexpression? I need the full protocol.

17 December 2014 10,025 3 View

Can anyone troubleshoot my URA3- selection problem?

I am trying to make URA3 mutant of an yeast strain. I have prepared FOA plates and when I carry out transformation using salmon sperm dna with my vector containing insert, kanamycin gene flanking...

29 June 2014 2,424 0 View