I have tried many times to amplify 16S fragment from genomic DNA but, unfortunately not succeeded.
I observed something awesome {Genomic DNA is viscous}
If your genomic DNA is contaminated with polysachharides, it do look viscous. did u carried out purification process? Try to purify twice. Then dilute the DNA in 1:10 or 1:100 ratio. Then proceed for 16S amplification.
Of Course, I did purification and I will do it again combining with dilution
Thanks
did you measure the concentration of DNA? if it is 2 or more ug/ul(viscous) then I would dilute first before making PCR.
Thanks for all, the PCR problems was solved after second purification using QIAGEN PCR purification kit combined with 4 times dillution.
Best Regards
Try diluting your genomic DNA 10 or 100 fold before you add it to the PCR reaction. Trust me, it will work.
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