Ganga Viswanath

3 Questions 21 Answers 0 Followers

Questions related from Ganga Viswanath

How can I completely remove DNA in my RNA sample?

I am isolating a total RNA from bacterial sample for real time PCR studies. But i am facing a problem with the DNA contamination. I treated the final RNA sample with DNases enzyme. I had done a...

09 September 2014 2,773 15 View

Does anyone know a selective medium for specific bacterial groups?

I am working in the isolation of specific groups of bacteria such as Stenotrophomonas, Cobetia, Sphingomonas, Artthrobacter from sediment and rhizosphere source. I have to be more specific in...

07 July 2014 3,742 0 View

How to overcome the problem in real-time PCR melting curve variation between the same replicates?

I have started with real time experiments. When I do a melting curve analysis for the primers, I get a different Tm for the same sample in the replicates. But there is no primer dimer, only single...

04 April 2014 6,004 16 View