How can I stop DNA annealing reaction?

11 December 2015 8,839 7 View

Why dose not a His-tag fused protein eluate during eluation step in FPLC even with > 2 M Imidazole concentration?

06 July 2015 3,764 12 View

What would be the reason for lower extinction coefficient of fluorescent protein than the expected one?

I purified a mRuby2 protein from Bactria. the extinction coefficient of this protein is 113000 M-1cm-1. but my expressed mRuby2 protein has a extinction coefficient of 45000 M-1cm-1. what would be...

02 March 2015 6,159 13 View

How can I express a gene in pBAD vector?

01 February 2015 2,014 10 View

How can I introduce of XbaI and Xho1 restriction sites at the end of my insert?

I want to insert a 423 bp gene at the end of my construct but I do not have any restriction site at the end. Therefore, I need to introduce two restriction sites to insert my gene. How can I...

02 March 2014 5,612 9 View

Anyone working with CLC Main Workbench program and knows how to add an annotation?

I have a sequence of my construct in CLC Sequence Viewer and I want to change the name of annotation and add another annotation to the construct sequence. Does anyone know how can I do...

06 July 2013 8,818 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View