Shivasankari Gomathinayagam

8 Questions 32 Answers 0 Followers

Questions related from Shivasankari Gomathinayagam

How to prevent clumping of EBV trasnfected lymphocytes?

I am culturing lymphocytes and they tend form visible clumps.. Does anybody know how to avoid this and if this is common ? I am new in culturing them. Thanks

05 May 2016 9,487 8 View

Could you share a protocol to culture lymphocytes ?

I am going to culture lymphocytes (EBV immortalized) for the first time and I am looking for protocols to help me with it. I don't see a lot of general culturing protocols online. Could you please...

04 April 2016 5,215 4 View

Contamination problems in 293 T cell culture. Any help ?

I have a contamination problem in the cell culture lab. The contaminant looks round, the size of yeast probably and visible under a light microscope (40X). Does not change the pH of the medium...

01 January 2016 1,926 17 View

Which antibody could I use against BRCA2 for Immunofluorescence?

I am looking for an Antibody to use in Immunofluorescence specific for BRCA2. I am not able to find a convincing IF image of BRCA2 by IF. Lot of scientists use the tagged version of BRCA2 in their...

09 September 2015 3,801 3 View

What are the ways to make the protein marker visible in a WB imager?

I have tried WB specific markers, but I would like to somehow image my protein markers while imaging. Is there a pen that would help ? Or are there other innovative ways?

05 May 2015 4,594 3 View

What is the conformation of the Holliday junction (HJ) inside the cell?

I am wondering if any of you have an idea about the conformation of HJ inside the cells - open or stacked? I am thinking it could be stacked, since that is a stable conformation than the open. But...

05 May 2014 5,665 2 View

How can I stabilise a protein-DNA complex in isolation?

I have purified my protein-Holliday junction complex by SEC, but the purified complex is not stable. The protein dissociates from the DNA. I would want the complex to be stable to perform further...

01 January 2014 3,248 3 View

Has anybody experienced protein sticking to the frits of the Superdex 200 column?

I am working on a human helicase protein and when I load them on a SEC column Superdex 200 (GE), I don't get a good recovery. In addition, when I load my protein-DNA complex into the same column...

01 January 2014 1,457 4 View