8 Questions 32 Answers 0 Followers
Questions related from Shivasankari Gomathinayagam
I am culturing lymphocytes and they tend form visible clumps.. Does anybody know how to avoid this and if this is common ? I am new in culturing them. Thanks
05 May 2016 9,500 8 View
I am going to culture lymphocytes (EBV immortalized) for the first time and I am looking for protocols to help me with it. I don't see a lot of general culturing protocols online. Could you please...
04 April 2016 5,230 4 View
I have a contamination problem in the cell culture lab. The contaminant looks round, the size of yeast probably and visible under a light microscope (40X). Does not change the pH of the medium...
01 January 2016 1,937 17 View
I am looking for an Antibody to use in Immunofluorescence specific for BRCA2. I am not able to find a convincing IF image of BRCA2 by IF. Lot of scientists use the tagged version of BRCA2 in their...
09 September 2015 3,810 3 View
I have tried WB specific markers, but I would like to somehow image my protein markers while imaging. Is there a pen that would help ? Or are there other innovative ways?
05 May 2015 4,608 3 View
I am wondering if any of you have an idea about the conformation of HJ inside the cells - open or stacked? I am thinking it could be stacked, since that is a stable conformation than the open. But...
05 May 2014 5,676 2 View
I have purified my protein-Holliday junction complex by SEC, but the purified complex is not stable. The protein dissociates from the DNA. I would want the complex to be stable to perform further...
01 January 2014 3,261 3 View
I am working on a human helicase protein and when I load them on a SEC column Superdex 200 (GE), I don't get a good recovery. In addition, when I load my protein-DNA complex into the same column...
01 January 2014 1,469 4 View