7 Questions 16 Answers 0 Followers
Questions related from Naghmeh Azadfar
I am annealing 37 DNA oligos together but i would like to stop it in certain time. i tried to add loading dye to stop it but it did not worked. Do you know how can i stop it?
12 December 2015 8,881 7 View
I am purifying a His-tag fused protein by Äkta instrument from GE Healthcare. The column in 1 ml HisTrap column. after loading the lysis to the column and washing by washing buffer, i elute the...
07 July 2015 3,838 12 View
I purified a mRuby2 protein from Bactria. the extinction coefficient of this protein is 113000 M-1cm-1. but my expressed mRuby2 protein has a extinction coefficient of 45000 M-1cm-1. what would be...
03 March 2015 6,196 13 View
I have a fluorescent protein in a pBAD vector and I want to express and purify it. At first, I cultured a colony in 100 ml LB medium at 37 °C for over night. Then, I inoculated 25 ml from over...
02 February 2015 2,064 10 View
I cultured my Insect cells at 27 °C and got yeast cell contamination. Which antibiotic should I use for insect cells culturing against yeast cells? Dose Pen-Strep work for that? How can I stop...
05 May 2014 4,381 3 View
I want to insert a 423 bp gene at the end of my construct but I do not have any restriction site at the end. Therefore, I need to introduce two restriction sites to insert my gene. How can I...
03 March 2014 5,669 9 View
I have a sequence of my construct in CLC Sequence Viewer and I want to change the name of annotation and add another annotation to the construct sequence. Does anyone know how can I do...
07 July 2013 8,897 1 View
