If I had 2 cell lines that different in their radius by 2 fold (one cell had a 5 micron radius, while the other had a 10 micron radius), but each cell had the same absolute number of receptors that were fluorescently labeled, how would these cells appear via:
1. Microscopy
2. Flow cytometry
I assume that via microscopy the larger cell would appear "half" as bright (since the receptor density is ~half), but via flow, the cells would appear to be the "same" brightness as each other (since as the cells pass by the detector, they would each have the same number of fluorophores emitting light).
Is that a good assumption?
Thanks,
Jim
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