How do we choose culture medium type; DMEM,MEM,RPMI?

Hello guys;I would like to learn how we can choose medium type. for example some papers say alpha-MEM is better than DMEM for human Mesenchymal Stem Cells (hMSCs) and some says DMEM is better than...

31 December 2014 2,163 26 View

How do B27 and insulin affect mammospheres?

I reached your paper but it is in Chinese so I could not understand it. Could you help me to understand how B27 and insulin affect MCF-7 derived mammospheres please? Thank you

02 March 2014 3,224 1 View

Does anyone have any idea what would be the best way to mimic Extracellular Matrix for mammospheres?

For the stiffness and if it is possible also the proteins. I found one product called Matrigel (BD Bioscience) but it has fenol red.. I need something transparent. I would appreciate if any of...

01 February 2014 7,334 5 View

Cholera toxin

I am going to work with MCF-7 derived mammospheres. I need cholera toxin. Most companies sends in within 2-4 months. Any suggestion where I can get it? I am in Italy.

06 July 2013 480 2 View

Can anyone share a basic protocol for non-adherent mammosphere ( derived MCF-7) culture?

I want to have a non-adherent mammosphere ( derived MCF-7) culture. Can anyone share a basic protocol, not with bioreactors or complicated scaffolds =) Grazie

05 June 2013 3,011 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View