How do we choose culture medium type; DMEM,MEM,RPMI?

Hello guys;I would like to learn how we can choose medium type. for example some papers say alpha-MEM is better than DMEM for human Mesenchymal Stem Cells (hMSCs) and some says DMEM is better than...

31 December 2014 2,163 26 View

How do B27 and insulin affect mammospheres?

I reached your paper but it is in Chinese so I could not understand it. Could you help me to understand how B27 and insulin affect MCF-7 derived mammospheres please? Thank you

02 March 2014 3,224 1 View

Cancer stem cells and nanomagnets

Could you suggest a paper that is about cancer stem cells and nanomagnets (nanocrystals..etc)?

06 July 2013 6,910 1 View

Cholera toxin

I am going to work with MCF-7 derived mammospheres. I need cholera toxin. Most companies sends in within 2-4 months. Any suggestion where I can get it? I am in Italy.

06 July 2013 480 2 View

Can anyone share a basic protocol for non-adherent mammosphere ( derived MCF-7) culture?

I want to have a non-adherent mammosphere ( derived MCF-7) culture. Can anyone share a basic protocol, not with bioreactors or complicated scaffolds =) Grazie

05 June 2013 3,011 3 View

No title

02 March 2021 3,060 3 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View