How could we participate in your project ?

More joan-lluis vives-corrons's questions See All

How can I improve the ligation fidelity of T4 ligase?

I am attempting to ligate an oligonucleotide with a gene that has a double-stranded DNA sequence. Approximately 10-20% of the DNA forms gene dimers instead of ligating with the oligonucleotide....

22 January 2024 3,310 2 View

How do I know if my lncRNA is polyadenylated or not?

I would like to know if my lncRNA is polyadenylated. To investigate this, I conducted an experiment using the same samples for generating cDNA using random primers in one set and oligo(dT) primers...

22 January 2024 7,106 0 View

How does Christina Sieloff's theory of group and power to the organization impact nursing safe staffing issues?

03 November 2023 6,124 1 View

What's the maximum passage number for T24 and RT4 bladder cancer cell lines?

Hello, does anyone know the maximum passage number recommended for T24 and RT4 bladder cancer cell lines? Thanks

10 October 2023 7,237 1 View

Intracellular calcium mobilization assay experiences in Varioskan LUX?

I am planning to perform intracellular Ca2+ mobilization assays on 96-well plates using EndoC-βH1 cells. Specifically, I am interested in measuring β-cell responses to glucose and possibly other...

16 July 2023 3,886 0 View

In working with a various anticancer medicinal plants , what are the best in vitro assays that can be employed to establish potency of the fraction?

in my study, using thses plants i have used bioassays, like mPT to establish the potency of the best solvent fraction to be subjected to further purification for identification and isolation of...

24 June 2023 8,737 1 View

What is the best approach for extraction, identification and complete isolation of anticancer bioactive agents from medicinal plants?

I will like to know the best approach to this because of the biodegradative nature of these bioactive compounds.

23 June 2023 4,087 1 View

How to prepare a wastewater microplastics samples for pyr-GC/MS?

Following digestion with Fenton's reagent, what is the best method to prepare a wastewater microplastics sample for pyr-GC/MS? It has been suggested that we filter the digested sample onto a PCTE...

11 June 2023 1,525 1 View

Hi! We are looking for comercially available printable ZnO inks for an ETL. Any tips?

Hi! We are looking for a printable ZnO ink for an electron transport layer. Preferably commercially available, doesn't matter the type (gel, nanoparticle-based, etc). We have find some but they...

22 January 2023 1,752 0 View

How important is serum-free medium for phagocytosis experiments?

Hello. Currently I am using the murine microglial cell line BV2 for phagocytosis experiments. I am wondering how key is the fact of treating the cells with serum-free medium. In what way affects...

13 October 2022 6,965 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View