Does anybody have a powerful Nvida Graphics card for collaborating in parallel simulations?

Dear college: I'm looking forward to find somebody who is having a powerful Nvida GPU for parallel GPGPU simulations in the context of GA's. I need to access remotely to a machine for running my...

13 April 2020 4,820 3 View

[Issue] What's wrong with my posterior distribution of the parameters in DIYABC?

I have an issue related to the posterior distribution of the parameters: the problem appears with two effective population sizes. It doesn't matter how high I write the prior interval (in the...

11 March 2020 2,989 1 View

What are the two important features present in an adult Enterobius vermicularis (pinworm)?

Give the two important feature that was discussed during laboratory.

10 March 2020 4,936 2 View

Is there any free, open source, softweare available to convert Agilent GC-MS (*.D) files to ANDI/AIA or JCAMP-DX?

Dear all, I am trying to convert some large Agilent GC-MS files (*.D) to a standard format such as ANDI/AIA (*.CDF) or JCAMP-DX (*.JDX). I was wondering if any one knows a piece of software that...

19 February 2020 8,289 3 View

Does anyone have reliable information to prepare a noninferiority trial?

Dear all, as you may know, in many clinical trials it is often not ethically acceptable to include a control group with no treatment. One option is to use a wait-list, but in many cases we end up...

18 February 2020 2,914 3 View

Is there a good antibody for a progenitor neuronal protein?

14 January 2019 10,019 4 View

Which is the best antibody to look for nucleolus degradation?

Hi everyone, I think that fibrillarin is the ideal one, but it would be really helpful to read about your experiences and suggestions. Thank you very much!

29 November 2018 7,133 2 View

How do I change something to show I published a review, not the original?

I was not given the option to specify I published a review, and am being asked if the author of the work I reviewed is a co-author. I have published over 50 reviews of the work of others.

05 November 2018 187 7 View

PCR product doesn't appear on gel but nanodrop suggested high concentration?

I did a standard KAPA HiFi Hotstart PCR reaction, including a positive control that I know has worked before in the cycling conditions I've used (basically what was recommended by the...

06 January 2017 6,453 7 View

How can I prepare a potasium-based fertilizer spray from banana peels and use it as a fertilizer getting rid of the biomass?

Specific objectives To extract potassium from banana peels using an acid To establish the elemental composition of the extract To modify the extracted liquid and formulate it into a liquid...

27 September 2016 2,695 12 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How to measure pH in anaerobic digester?

I'm planning on doing research by using anaerobic digester with liquid substrate and want to see its pH changes periodically. Any suggestion on measuring the pH with maintaining its anaerobic...

09 February 2021 1,293 1 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View