Hi,

I would like to transfect Jurkat T cells with some plasmids. So far I have used commercial reagents and every time got to see transfection efficiency nearly 70-80%. However, since these reagents are very costly and I need to do a lot of transfections in the coming days, my boss asked to me to try some home made solutions, that would be much cheaper. We have a gene pulser electroporator from BioRad for doing the electroporation. Could any one tell me the recipe for any of such home made reagents and the protocol that I can use?

Thanks