Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

MOS Capacitor instance or a mosfet used as a cap (D-S-B connected to gnd)?

Hi, in Cadence Spectre simulations should I use a MOS cap instance or a mosfet (D-S-B connected to gnd) to simulate a real life MOS-cap? I tried both of them and they gave different results i.e....

26 December 2020 6,407 1 View

Angiotensin peptide stability?

Hello all, We are currently working on angiotensin analysis, however we observed that, once the freeze-dried peptide is dissolved in an aqueous buffer, it gets unstable in only 2-3 hours. Mass...

13 November 2019 787 1 View

Can anyone suggest me how to approach to design a Frequency Hopped OFDM system on Simulink?

The proposed system deals with wideband frequency hopping. The system will be able to hop between three sub-bands, with each sub-carrier having a bandwidth of 40 MHz. The three sub-bands hopping...

08 November 2019 2,574 2 View

How can I isolate mitochondrial DNA from adherent cell lines?

I have tried using the Plasmid isolation Kit as recommended by...

22 October 2019 2,118 6 View

Can microbiology pipettes be used for cell culture?

Hello everyone, We have a multichannel pipette in the microbiology lab that has been used against various pathogenic bacteria. If we want to use it for cell culture, can it be sterilised? If yes,...

08 August 2018 9,873 14 View

Ligation Reaction for cloning?

Is it always a good idea to go for quick ligation reaction instead of normal ligation procedure. I was wondering at what fundamental base we decide to go for quick ligation or normal ligation...

17 June 2018 161 3 View

I see artifacts or bands in SDS-PAGE gel in lanes I have not loaded any sample, I have changed acrylamide, SDSPAGE loading dye, any suggestions?

I have changed SDS-PAGE loading dye, acrylamide, gel running buffer , running short of ideas no, please help

04 February 2018 8,514 6 View

Can you suggest me a high electrical conductive material which i can use at 1100 C in H atmosphere

I tried graphite before but i faced ash like wearing problem. I made some research and found Inconel 601 - 617 (Nickel alloy) and TMZ alloy (Molybdenum alloy) which are hard to reach. I will try...

02 August 2017 6,741 3 View

Stress-Strain damping-strain curves for different soil medium?

14 July 2017 6,805 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View