what exactly are you asking? You can isolate total RNA by standard procedures , than enrich polyA RNA on polyT affinity beads and sequence. The question is what technology of sequencing you will use, you will have to prepare the sample based on that.
I think that if you have a good yield almost every RNA isolation technique may work (total RNA, micro RNA if you are interested). Do you use a kit or a classical trizol + cloroform/ethanol extraction?
Also it depends if you want to focus on one type of RNA: if you want to enrich in mRNA transcripts you can use oligo-dT Beads (as Lucia Hronska commented), if you want to focus on microRNAs or tRNAs you may use a micro-RNA isolation kit, if you want ribosomal RNA you can perform a gradient centrifuge (with sucrose for instance) and then isolate the enriched eluted fractions and later peform the RNA isolation (Ref: Liang S, Bellato HM, Lorent J, Lupinacci FCS, Oertlin C, van Hoef V, Andrade VP, Roffé M, Masvidal L, Hajj GNM, Larsson O. Polysome-profiling in small tissue samples. Nucleic Acids Res. 2018 Jan 9;46(1):e3. doi: 10.1093/nar/gkx940)
What sequencing technique will you use?? And in what kind of RNA are you interested?
Best regards!
Francesc
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