Pretreated lignocellulose releases sugars during "Enzymatic hydrolysis" and those sugars can be used to produce biofuels. Can someone explain how can we determine the "amount of buffer" which needs to be added to the reaction mixture containing pretreated biomass and crude enzymes?
Hello well, it depends of the liquid solid ratio, in our laboratory we did experimental desing for each lignocellulosic waste, and with the liquid solid ratio (LSR), we can determin what is the amoung of buffer.
for example, in this article the LSR optimum value was 5, then, per each gr of cellulose you use 5 ml of buffer.
The amount of buffer is a process choice. If you choose 10% solids in your process, you will end up with a more dilute hydrolyzate. If you choose higher solid loads you will have a more concentrated hydrolyzate. Choice alternatives influence other steps such as separation.
That said, when determining your process you will need to know the moisture content of your biomass to calculate solids content always on a dry basis and the the enzyme load you want and finally the buffer mass/volume.
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