Muhammad Jawad

9 Questions 18 Answers 0 Followers

Questions related from Muhammad Jawad

What strategies would you adopt to maximize cellulase activity per gram of biomass produced?

This could involve manipulating media composition, fermentation conditions (pH, temperature, oxygen), or even strain engineering approaches.

13 February 2024 2,121 1 View

How to maximize cellulase production from Trichoderma reesei?

What specific carbon source and environmental conditions have been found to be most effective in enhancing cellulase production by Trichoderma reesei, and what molecular mechanisms are believed to...

31 July 2023 2,049 1 View

How is Chat GPT created and how powerful it is?

Currently its free and available to almost everyone. What would be its future and will it be free forever? Furthermore, how powerful it is in terms of developing new ideas?

21 January 2023 1,654 16 View

What is the easiest way to learn Response Surface Methodology (RSM)?

I am planning to work on optimization of different parameters in my studies. I want to learn and apply RSM. Could someone please suggest or recommend some easy papers and softwares?

20 January 2022 1,240 3 View

What is the most advanced and efficient pretreatment for Corn stover?

Corn stover is a cheaper lignocellulose that is used to produce biofuels. There are numerous methods for pretreatment of corn stover before hydrolysis. Among those, which one is the most efficient?

11 December 2021 3,068 3 View

How can you calculate the amount of buffer needed for an enzymatic hydrolysis reaction?

Pretreated lignocellulose releases sugars during "Enzymatic hydrolysis" and those sugars can be used to produce biofuels. Can someone explain how can we determine the "amount of buffer" which...

14 September 2021 7,000 3 View

How to convert cellulase enzyme production (FPU/mL) to productivity (FPU/L/h)?

Hello every one, I am working on cellulase enzyme production. If I achieve 10 FPA/ml in 12 hours, how would I convert this value to g/L/h? Is it correct if I divide 10 by 12 and then multiply by...

26 July 2021 458 2 View

Why do I get bands (Less than 100 bp) and extremely light target bands which range from 1.4 to 1.8 Kb?

I am running gel electrophoresis to confirm multiple target genes from PCR (not multiplex). Gene sizes range from 1.4 kb to 1.8 kb and the annealing temperature is 59 C. The same PCR reaction...

05 March 2021 4,410 6 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

02 March 2021 776 7 View