How can we selectively enumerate Lactobocillus plantarum in a medium containing Lactobacillus plantarum and Lactobacillus acidophilus?

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What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

What is the best way to do symmetry in ANSYS Fluent for flow between two concentric cylinders ?

I am working on Taylor Couette flow between concentric cylinders and the results are correct for the full case. however, I wanted to do symmetry case to improve the mesh. I have tried periodic...

11 February 2021 2,193 6 View

What is the success rate of the e-learning plan in achieving the objectives of the curricula?

I need to know the reliability of the evaluation results and the rate of achievement of the course objectives, Thank you (*-*).

02 February 2021 7,828 7 View

What is the success rate of the e-learning program in your country during the time of Covid-19?

I need to know the extent of the success of the e-learning plan in countries of different capabilities, Thank you (*-*)

02 February 2021 4,712 15 View

How can I get good bands by silver nitrate staining on SSCP method?

Hi Dear colleagues This was my first experience on SSCP, and I couldn`t get any band by silver staining! Staining with Et Br was successful. I mixed the PCR product with formamide loading dye,...

01 February 2021 8,788 6 View

Why Selenium nanoparticles synthesized by PEG 200 could not be scaled up ?

I could not synthesize Selenium nanoparticles by PEG 200 at up-scale ( more than 100 ml) as it got aggregated while at small amounts it works fine , I work with 850 PPM using selenium powder in...

08 January 2021 10,049 1 View

Solving a third order polynomial matrix equation?

I want to solve the following matrix equation with respect to the matrix variable X which is a real symmetric positive definite matrix. The given matrix A is real and symmetric, "a" is a scalar,...

04 January 2021 2,945 7 View

What is the best method to add nanoparticles in organic solar cell simulation using silvaco software ??

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03 January 2021 7,107 11 View

How to Calculate Local and Average NUSSELT NUMBER IN FLUENT ANSYS ?

I am simulating a single flow in a 2D tube with a uniform heat flux applying on the outer surface. I found difficulties finding the Nusselt Number or in other words a correct way to obtain...

26 December 2020 4,054 7 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Why there is no increase in virus titer after 24 hour of seeding for harvest?

I am working on virus production through bioreactor,my concern is regarding ,no increase in virus titer after 24 hour of seeding for harvest Usually we seed the fermenter and incubate for 72...

05 January 2021 3,860 2 View

How to extract trypsin inhibitor from fermented samples ?

Hi. I need help to measure trypsin inhibitor activity in fermented soy and fermented oat samples. The problem is my samples were mixed with water with ratio 1:3 and 1:2 respectively, for...

13 December 2020 6,882 3 View

What is the principle behind the delayed in the production of acid from lactose in late lactose fermenters?

Explain the principle behind the delayed production of acid

19 October 2020 3,251 4 View

What is the best pH to grow Lactobacillus rhamnosus?

Hi all, I'm doing some research in the proteins secreted by L rhamnosus and I've been trying to optimise the fermentation conditions to maximise production of these proteins. Without pH control...

08 October 2020 4,647 6 View

Why Lactobacillus acidophillus is sensitive to Vancomycin but L. rhamnosus and casei are intrinsically resistant?

Does it have, anything to do with their fermentation status? I know that in several species of LAB, the terminal d-alanine residue is replaced by d-lactate or d-serine in the muramylpentapeptide,...

19 September 2020 3,242 2 View

Acceptable CV% and SD for results between stool samples in qPCR?

What would be the acceptable CV% and SD for copy number results between stool samples in qPCR (same group)? I am evaluating different bacteria (E.coli, Lactobacillus, etc.) using the absolute...

06 August 2020 8,112 4 View

I am looking for this strain, Lactobacillus acidophilus (strain for minicells production). Please can you help me to find it?

I am a master student, I need this strain for research purposes. Lactobacillus acidophilus [ minicells producing strain (Minicells are generally produced by aberrant cell divisions at...

13 March 2020 3,204 3 View

Alaa Kareem Niamah

You can anther selective media I sent you two paper about this subject

Mona Mahmoud

Thanks a lot, Dr. Michael

Mohamed El-ssayad

Try to reconstitute MRS considering the sugar type that can be utilized by L. plantarum, while L. acidophilus cannot utilize it.

Babar Hilal Ahmad Abbasi

Grateful, I got valuable information too