Can a lactic acid bacterium have two different microscopic forms (bacillary form and a supercoiled form)?

25 April 2024 0 6K Report

The culture was pure (there was one colony type on MRS solid medium). I did Gram staining on one colony and I found the two morphological types. Many trials were carried out and I got the same results each time.

Badges
Science topic

More Nabila Brahamia's questions See All

What are the causes of sexual harrasment at sports setting?

causes behind the harrasment thinking towards females athletes, and how these could be measures

03 January 2020 7,289 3 View

Fuzzy rule based system: Should rules contain all inputs and outputs?

Dear all, I am trying to design an FRBS using Matlab fuzzy logic toolbox. The fuzzy system will be used to predict player's type based on inputs (gameplay data) and a set of rules defined by...

27 December 2018 5,711 7 View

What algorithm to use to classify users based on collected metrics and a set of rules?

I have a data set that contains in-game players actions and interactions (stored as metrics). I want to create a recognition system that contains a set of predefined rules and takes as inputs the...

10 April 2018 9,494 5 View

We are using UV Spectroscopy to identify DNA Damage cultured in Broth containing formalin. What methods can be used for identifiying the damage?

We have cultured E. coli in Broth containing formalin. We also measured the growth concentration by Uv Spectroscopy. Our target is to identify DNA damage and DNA mutation location.

24 February 2018 7,546 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View