Is there any available dataset that has COVID-19 symptoms values ?

I need a dataset which has real values of COVID-19 patients symptoms such as cough and temperature? I have found some datasets online but it contains only 0 or 1 for the symptoms values? Anyone...

29 January 2021 2,232 3 View

Dynamics of cochlea membrane ?

04 November 2020 3,333 1 View

I run GSSHA model in WMS software but the result was not reasonable what is the problem?

i do it with 32 mm precipitation

05 August 2019 8,187 2 View

What is the best formulation to disperse a commercialized graphene in DMF?

I want to prepare a conductive ink. But i couldn't find any journal that provides a formulation for dispersing commercialized graphene in DMF. I want to know what formulation can give a homogenous...

20 March 2019 5,757 7 View

Bacteria E.Coli have Fluorescence when dissolved in Phosphate buffer and DI water???

I want to capture bacteria with Carbon dots, but i get flourescence intensity like jumping up and down by UV, and then i am confuse, why like that? or may be i need to measure OD 600 and...

15 August 2018 3,097 3 View

Dose anyone know how we analysis LC-MS data please?

I have run my crude extracted in LC-MS and i do not know how I analysis it.

16 February 2018 2,403 3 View

What is the minimum number of informants to calculate the informant consensus factor (Fic), UV, FL and others in quantitave ethnobotanical techniques?

I have only 8 key informants because I selected them based on certain criteria. However, I would like to analyze the data quantitatively. Thanks in advance.

20 September 2015 5,508 15 View

How to report the results of Intra-Class Correlation Coefficient Results?

If I have the results as attached, how do I report it in presentation slides?

18 September 2014 504 6 View

Can you help me with a problem on reliability statistics?

My Cronbach Alpha stated -0.656. The note below says: The value is negative due to a negative average covariance among items. This violates reliability model assumptions. You may want to check...

11 July 2014 9,920 16 View

Can anyone help me on SPSS?

I am still new in SPSS. If I wanted to include this question: 'How many students are in your classes, by gender?' as a variable/variables, do I need to compute 'students', 'classes' and 'gender'...

22 April 2014 762 16 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View