Chloroform saturated PBS
The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.
23 July 2024 3,205 0 View
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,284 4 View
I want to make 100 ml of MWCNT/distilled water mixture at a volume fraction of % 0.1. MWCNT density is 2100 kg/m3. I will add gum arabic as surfactant. If I add surfactant at a 1:1 ratio, will the...
19 July 2024 8,494 0 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
What is the future scope of acoustic emission?
17 July 2024 1,510 1 View
I have a small molecule reagent with a boc-protected amino group. Now the reaction needs to be reacted at 37°C for 30 h. Is this protection group stable?
12 July 2024 3,745 2 View
I am trying to measure the plasmon resonance angle of gold film and pure water using the Kretschmann configuration and a 633nm laser. Without flowing water over the gold, I can detect a clear...
10 July 2024 4,719 3 View
Hello! I am facing a problem, I tried using pdb2gmx to generate topology file but it shows error HEA residue not found then I tried copying the forcefield file and edited accordingly but again...
24 June 2024 1,242 0 View
Could anyone suggest me what is effect of electron donating and withdrawing group on fluorecence emission or intersystem crosssing. Thank you
22 June 2024 2,656 2 View
I submitted my journal and got some comments, I have answered and corrected all the comments from the reviewer but I don't understand how to answer this question. any expert researcher help me to...
20 June 2024 3,802 1 View
After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...
29 July 2024 9,753 3 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
I need to do a Schiff base reaction starting from a ketone and 1,10-phenanthroline-5-amino. I've seen that both of these starting materials are not very soluble in methanol and ethanol, which is...
23 June 2024 5,925 3 View
and chloroformate. But it takes 10 days to compete the reaction. How can I shorten the time of the reaction?
14 June 2024 2,924 3 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
Greetings We are trying to study Cholesteryl esters. We start with MeOH/Chloroform extraction and then remove most of the noise by silica column, but the Cholesteryl esters come out with...
02 June 2024 5,545 0 View
We have been trying to extract RNA from brown algae. We have been following traditional CTAB method as modified by Sim et al., 2013. We get a very thick white jelly interface with the Chloroform...
30 May 2024 356 0 View
I've been working off an RNA extraction protocol using trizol and chloroform for several months that worked beautifully until I moved on to our most important samples. After completing them, I...
23 May 2024 2,475 1 View
Im extracting RNA from tomato leaves by the TRIZOL method but since the chloroform phase separation I can see that the aqueus phase is brownish and the pellet is totally brown. When I evaluate the...
21 May 2024 9,910 1 View
For my project, I need to extract RNA from muscle tissue of EEL. I am trying but the yield is low and Purity not good. The 260:230 ratio is always below 1.50 and 260:280 ratio ranges 1.6-1.86 and...
15 May 2024 3,024 2 View
