Hi all -
I was wondering how one could 'easily' identify the receptor of a small molecule in bacteria. Background: we identified a small molecule that inhibits biofilm formation in Salmonella and we wonder, which protein(s) are potential receptors. I assume one can do a genetic screen but can we also do a biochemical approach e.g. can we chemically couple the compound to a resin and add whole cell proteins to resin to see which proteins bind and analyze them afterwards. I assume many people do this kind of research and I wonder what are the simplest approaches.
Thank you and have a nice day
Kornelius
The experiment you describe is known as a "pull-down."
Here are a few papers describing variations, and a commercial web site:
https://www.pnas.org/content/106/12/4617
Article Identification of Direct Protein Targets of Small Molecules
Article Identification and validation of protein targets of bioactiv...
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/pull-down-assays.html
This technique is named Pulldown, the best and easy approach to find an unknown protein/s from crude extract for your protein of interest ...
Good luck
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