Are there any data/program/server available to predict/approximate the running behaviour (peak retention) of a needle like protein (7 x 2 x 2 nm) on a size exclusion column.
Thanks and best wishes
Kornelius
Hi Kornelius, interesting problem! Size exclusion will not work for needle-like proteins.
You could try anion/cation exchange columns to see where the smallest,/biggest fractions come out. Hydrophobic resins might be other alternative. Let me know,
Stenni
Non-globular proteins can be analyzed by gel filtration chromatography, but may elute differently than a globular protein of the same mass. I don't know if there are any on-line sources for predicting elution volume.
This book is an excellent reference on gel filtration:
http://labs.mcb.harvard.edu/Gaudet/Resources_Files/GEHealthcare_chromatography/Don't%20move/18102218.pdf
Agree with Adam on this one, it is possible to separate fibrous proteins, but their elution volume/time will differ from globular proteins.
And due to their structure you can expect a lot of dispersion leading to broader peaks.
Hi Kornelius,
you may want to look at Wang et al. 2010 Macromolecules 43,1651-1659.
All the best
Matthias
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