12 December 2016 10 8K Report

Dear all, 

We would like to check the purity of a GST-tagged recombinant protein by SDS-PAGE. We purified the protein using Baculovirus expression system.

We run the reducing SDS-PAGE (GST was used as a positive control) to check for purity. But unfortunately, we can only see a small band of our target protein (the expected molecular weight: 65 kDa) except for GST and some cleavages. 

I don't know whether it was due to the reducing SDS-PAGE that we used or the protein purification has failed? If it is possible, I hope to receive any of your comments or suggestion. 

Thank you, 

Yours sincerely, 

Tu

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